Tuberculosis is a highly infectious disease with about one third of the world’s population including 40 per cent from India estimated to be infected it (1). However, this problem has become serious as Mycobacterium tuberculosis developed resistance against both the first line as also the second line drugs. Due to this, there is emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) strains of M. tuberculosis all over the world including India (2). Medicinal plants offer a great hope to fulfill these needs and have been used for curing diseases for many centuries. These have been used extensively as pure compounds or as a crude material. Only a few plant species have been thoroughly investigated for their medicinal properties (3). India is one of the few countries in the world which has unique wealth of medicinal plants and vast traditional knowledge of use of herbal medicine for cure of various diseases (4,5). So far, few plants have been tested against mycobacteria and a few plants which showed anti-TB activity were Salvia hypargeia, Euclea natalensis, etc. (6,10). The increasing incidence of MDR- and XDR-TB worldwide highlight the urgent need to search for newer anti-tuberculosis compounds/ drugs. Therefore, the present study was carried out to check the antibacterial activity of aqueous extracts of five plants against MDR isolates of M. tuberculosis, reference susceptible strain M. tuberculosis H37Rv as well as fast growing mycobacterial pathogen M. fortuitum (TMC-1529). Plants already reported to have anti-tuberculosis activity (Adhatoda vasica (11,12), Allium cepa (13,14) and Aloe vera 15-17) were selected to test their activity further against MDR strains of M. tuberculosis, while selection of Acalypha indica (18) was based on its ethno-medicinal uses in respiratory disorder. A. cepa was selected on the basis of knowledge that A. sativum has anti-tuberculosis activity; therefore other species of Allium might also have anti-tuberculosis activity.