Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)

Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)

Background & objectives: Glycoprotein B (gB), involved in ceil-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. Methods: The PCR based RFLP and the multiplex nested PCR were applied on standard strain of HCMV AD169, 4 clinical HCMV isolates and 70 clinical specimens positive for HCMV by pp65 antigenaemia assay or nested PCR for mtr II region or both.

Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)

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