DNA Sequencing by Microseq Kit Targeting 16S Rrna Gene for Species Level Identification of Mycobacteria (Report)

DNA Sequencing by Microseq Kit Targeting 16S Rrna Gene for Species Level Identification of Mycobacteria (Report)

Background & objectives: Identification of mycobacteria to the species level is of therapeutic significance. Conventional methods are laborious and time consuming so we did 16S rRNA sequencing using a commercial MicroSeq sequencing kit, which includes DNA sequencing with software package for identification and phylogenetic analysis of clinical mycobacterial isolates. Methods: A total of 47 mycobacteria were tested by both conventional and genotypic method using commercially available MicroSeq 500 amplification kit assay. The identification was determined by comparing the 500 bp amplified product of 16S rDNA sequence to the MicroSeq database.

DNA Sequencing by Microseq Kit Targeting 16S Rrna Gene for Species Level Identification of Mycobacteria (Report)



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