Establishment of T-Lymphocyte Subset Reference Intervals in a Healthy Adult Population in Chennai, India (Report)

Establishment of T-Lymphocyte Subset Reference Intervals in a Healthy Adult Population in Chennai, India (Report)

Estimation of CD4+ T-lymphocytes continues to be an important aspect for monitoring HIV disease progression and response to antiretroviral therapy. Most of the diagnostic laboratories often rely on western text books for CD4+ T-lymphocyte reference values, which could, often be unreliable for usage in local settings. Therefore, we attempted to establish the reference values for T-lymphocyte subsets among healthy adults in a cross-sectional study carried out at the YRG Centre for AIDS Research and Education (YRG CARE) in Chennai, south India, in 213 (84 female and 129 male) healthy, HIV-1/2 seronegative adults as volunteers. Whole blood specimens were processed for CD4+, CD8+ T-lymphocyte estimation and haematological parameters. The established range of CD4+ T-lymphocyte counts for men and women were 383-1347 cells/[micro]l (mean 865 and median 845 cells/[micro]l) and 448-1593 cells/[micro]l (mean 1021 and median 954 cells/[micro]l), respectively. Women had significantly higher absolute CD4+ T-lymphocyte counts (P0.001) and CD4+:CD8+ T-lymphocyte ratio as compared to men. The established normal range of CD4+ T-lymphocyte % was 21-59 (mean 40.2 and median 40.1). The influence of age was not observed in any of the parameters except CD4+/CD8+ T-lymphocyte ratio with the 45 yr age group. Further studies with greater sample size may be required to define the staging of HIV disease in relation to the normal CD4 T-lymphocyte count in the general population. Key words CD4+ T-lymphocyte–India–reference range

Establishment of T-Lymphocyte Subset Reference Intervals in a Healthy Adult Population in Chennai, India (Report)

Establishment of T-Lymphocyte Subset Reference Intervals in a Healthy Adult Population in Chennai, India (Report) | | 4.5